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- Heaters/Source
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- Agilent Heaters and SensorsMass Spectrometry, Scientific Supplies & ManufacturingScientific Instrument Services 5973 Source Heater Tamper Resistant Allen Wrench 5973/5975 Quad Sensor 5985 Source Heater Assembly Agilent Interface Heater Assembly 5971 Interface Heater
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- Reference Material on InstrumentationArticle - A High Temperature Direct Probe for a Mass Spectrometer Design of a Direct Exposure Probe and Controller for use ona Hewlett-Packard 5989 Mass Spectrometer SIS AP1000 AutoProbe™ SIS AP2000 AutoProbe™ - Description of System HPP7: Direct Probe Electronics Console HPP7: Direct Probe for the Agilent (HP) 5973/5975 MSD HPP7: HP Direct Probe Application Notes HPP7: Installation Directions for the Direct Probe HPP7: Side Cover for the HP 5973 MSD HPP7: Support HPP7: Probe Inlet System for the Agilent (HP) 5973 and 5975 MSD with Automatic Indexed Stops HPP7: Theory of Operation of the Direct Probe and Probe Inlet System Direct Thermal Extraction Thermal Desorption Application Notes Environmental Thermal Desorption Application Notes Food Science Thermal Desorption Application Notes Forensic Thermal Desorption Application Notes GC Cryo-Trap Application Notes Headspace Application Notes Purge & Trap Thermal Desorption Application Notes Theory of Operation of the AutoDesorb® System AutoDesorb Notes for SIS Dealers Adsorbent Resin Application Notes Installation of the Short Path Thermal Desorption System on Agilent (HP) and Other GCs Installation of the Short Path Thermal Desorption System on a Varian 3400 GC AutoDesorb® System Development Team Thermal Desorption Applications and Reference Materials Installation of the Short Path Thermal Desorption System - TD5 Part I - Design & Operation of the Short Path ThermalDesorption System Installation Instructions for the Model 951 GC Cryo-Trap on the HP 5890 Series GC Installation Instructions for the Model 961 GC Cryo-Trap on the HP 5890 Series GC Operation of the Model 951/961 GC Cryo-Trap SIS GC Cryo Traps - Theory of Operation NIST/EPA/NIH Mass Spectral Enhancements - 1998 version (NIST98) SIMION 3D Ion Optics Class Mass Spectrometer Source Cleaning Methods MS Tip: Mass Spectrometer Source Cleaning Procedures Mass Spec Source Cleaning Procedures Micro-Mesh® Abrasive Sheets Research Papers Using New Era Syringe Pump Systems EI Positive Ion Spectra for Perfluorokerosene (PFK) Cap Liner Information How do I convert between fluid oz and milliliters? Which bottle material should I choose? Which bottle mouth should I choose? The Bottle Selection Guide CGA Connections for Gas Tanks Chemical Reaction Interface Mass Spectrometry (CRIMS)
- Instrument Tubing
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- Gas RegulatorsModel 3530 Series - Single Stage Purity Brass Regulator Model 3510 Series - Single Stage High Purity Stainless Steel Regulators Model 3120 Series - Dual Stage Purity Brass Regulator Model 3810 Series - Dual Stage High Purity Stainless Steel Regulators Tescom Gas Line Regulators 3420 Series Tescom Gas Line Regulators 3450 Series Concoa In-Line Regulators Model 304 Series Concoa In-Line Regulators Model 324 Series
- TD
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- AccessoriesTD Supply Kit Desorption Tubes Adsorbent Resins Desorption Tube Needles Desorption Tube Seals Desorption System Fittings GC Cryo-Trap Extraction Cell TD Sample Loader Prepacked, Conditioned Desorption Tubes Desorption Tube Packing Accessories Stainless Steel Purge Heads Injection Port Liners Tenax TA Poster TD Application Notes Customer Service
- GCColumns Fused Silica Tubing Instrument Tubing Injection Port Liners Septa by Manufacturer SIS GC Cryo-Traps Ferrules Valves Swagelok® Fittings Pyrolysis Probe Accessories Gas Generators Gas Regulators Gas Purifiers and Filters Syringes SGE MEPS™-Micro Extraction by Packed Sorbent Purge and Trap System SGE SilFlow™ Stainless Steel Micro-Fluidic Platform Accessories NIST GC RI Library Other GC Supplies Catalog Page D1
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- GC Cryo-traps
- LiteratureApplication Notes Adsorbent Resins Guide Mass Spec Tips SDS Sheets FAQ MS Calibration Compound Spectra Manuals MS Links/Labs/ Organizations MS Online Tools Flyers on Products/Services Scientific Supplies Catalog About Us NextAdvance Bullet Blender® Homogenizer Protocols Micro-Mesh® Literature Instrumentation Literature Agilent GC/MS Literature SIS News / E-Mail Newsletter NIST MS Database - Update Notifications
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- Thermal Desorption Applications and Reference MaterialsDirect Thermal Extraction Headspace Environmental Food Science Applications Pharmaceuticals Forensic Note 103: EPA Method 325B, Novel Thermal Desorption Instrument Modification to Improve Sensitivity Note 102: Identification of Contaminants in Powdered Beverages by Direct Extraction Thermal Desorption GC/MS Note 101: Identification of Contaminants in Powdered Foods by Direct Extraction Thermal Desorption GC/MS Note 100: Volatile and Semi-Volatile Profile Comparison of Whole Versus Cracked Versus Dry Homogenized Barley Grains by Direct Thermal Extraction Note 99: Volatile and Semi-Volatile Profile Comparison of Whole vs. Dry Homogenized Wheat, Rye and Barley Grains by Direct Thermal Extraction GC/MS Note 98: Flavor and Aroma Profiles of Truffle Oils by Thermal Desorption GC/MS Note 97: Flavor Profiles of Imported and Domestic Beers by Purge & Trap Thermal Desorption GC/MS Note 95: Detection of Explosives on Clothing Material by Direct and AirSampling Thermal Desorption GC/MS Note 94: Detection of Nepetalactone in the Nepeta Cataria Plant by Thermal Desorption GC/MS Note 93: Detection of Benzene in Carbonated Beverages with Purge & Trap Thermal Desorption GC/MS Note 88: Analysis of Silicone Contaminants on Electronic Components by Thermal Desorption GC-MS Note 84: Vacuum Pump Exhaust Filters - Charcoal Exhaust Traps Note 83: Vacuum Pump Exhaust Filters - Oil Mist Eliminators Note 82: Vacuum Pump Exhaust Filters Note 80: Design, Development and Testing of a Microprocessor ControlledAutomated Short Path Thermal Desorption Apparatus Note 79: Volatile Organic Compounds From Electron Beam Cured and Partially Electron Beam Cured Packaging Using Automated Short Path Thermal Desorption Note 77: The Determination of Volatile Organic Compounds in VacuumSystem Components Note 75: An Apparatus for Sampling Volatile Organics From LivePlant Material Using Short Path Thermal Desorption Note 73: The Analysis of Perfumes and their Effect on Indoor Air Pollution Note 71: Flavor Profile Determination of Rice Samples Using Shor tPath Thermal Desorption GC Methods Note 65: Determination of Ethylene by Adsorbent Trapping and Thermal Desorption - Gas Chromatography Note 64: Comparison of Various GC/MS Techniques For the Analysis of Black Pepper (Piper Nigrum) Note 63: Determination of Volatile and Semi-Volatile Organics in Printer Toners Using Thermal Desorption GC Techniques Note 60: Programmable Temperature Ramping of Samples Analyzed ViaDirect Thermal Extraction GC/MS Note 57: Aroma Profiles of Lavandula species Note 55: Seasonal Variation in Flower Volatiles Note 54: Identification of Volatile Organic Compounds in Office Products Note 43: Volatile Organic Composition In Blueberries Note 42: The Influence of Pump Oil Purity on Roughing Pumps Note 41: Hydrocarbon Production in Pine by Direct Thermal Extraction Note 40: Comparison of Septa by Direct Thermal Extraction Note 39: Comparison of Sensitivity Of Headspace GC, Purge and Trap Thermal Desorption and Direct Thermal Extraction Techniques For Volatile Organics Note 38: A New Micro Cryo-Trap For Trapping Of Volatiles At the Front Of a GC Capillary Column Note 37: Volatile Organic Emissions from Automobile Tires Note 36: Identification Of Volatile Organic Compounds In a New Automobile Note 35: Volatile Organics Composition of Cranberries Note 34: Selection Of Thermal Desorption and Cryo-Trap Parameters In the Analysis Of Teas Note 33: Changes in Volatile Organic Composition in Milk Over Time Note 32: Selection and Use of Adsorbent Resins for Purge and Trap Thermal Desorption Applications Note 31: Volatile Organic Composition in Several Cultivars of Peaches Note 30: Comparison Of Cooking Oils By Direct Thermal Extraction and Purge and Trap GC/MS Note 29: Analysis Of Volatile Organics In Oil Base Paints By Automated Headspace Sampling and GC Cryo-Focusing Note 28: Analysis Of Volatile Organics In Latex Paints By Automated Headspace Sampling and GC Cryo-Focusing Note 27: Analysis of Volatile Organics In Soils By Automated Headspace GC Note 26: Volatile Organics Present in Recycled Air Aboard a Commercial Airliner Note 25: Flavor and Aroma in Natural Bee Honey Note 24: Selection of GC Guard Columns For Use With the GC Cryo-Trap Note 23: Frangrance Qualities in Colognes Note 22: Comparison Of Volatile Compounds In Latex Paints Note 21: Detection and Identification Of Volatile and Semi-Volatile Organics In Synthetic Polymers Used In Food and Pharmaceutical Packaging Note 20: Using Direct Thermal Desorption to Assess the Potential Pool of Styrene and 4-Phenylcyclohexene In Latex-Backed Carpets Note 19: A New Programmable Cryo-Cooling/Heating Trap for the Cryo-Focusing of Volatiles and Semi-Volatiles at the Head of GC Capillary Columns Note 18: Determination of Volatile Organic Compounds In Mushrooms Note 17: Identification of Volatile Organics in Wines Over Time Note 16: Analysis of Indoor Air and Sources of Indoor Air Contamination by Thermal Desorption Note 14: Identification of Volatiles and Semi-Volatiles In Carbonated Colas Note 13: Identification and Quantification of Semi-Volatiles In Soil Using Direct Thermal Desorption Note 12: Identification of the Volatile and Semi-Volatile Organics In Chewing Gums By Direct Thermal Desorption Note 11: Flavor/Fragrance Profiles of Instant and Ground Coffees By Short Path Thermal Desorption Note 10: Quantification of Naphthalene In a Contaminated Pharmaceutical Product By Short Path Thermal Desorption Note 9: Methodologies For the Quantification Of Purge and Trap Thermal Desorption and Direct Thermal Desorption Analyses Note 8: Detection of Volatile Organic Compounds In Liquids Utilizing the Short Path Thermal Desorption System Note 7: Chemical Residue Analysis of Pharmaceuticals Using The Short Path Thermal Desorption System Note 6: Direct Thermal Analysis of Plastic Food Wraps Using the Short Path Thermal Desorption System Note 5: Direct Thermal Analysis Using the Short Path Thermal Desorption System Note 4: Direct Analysis of Spices and Coffee Note 3: Indoor Air Pollution Note 2: Detection of Arson Accelerants Using Dynamic Headspace with Tenax® Cartridges Thermal Desorption and Cryofocusing Note 1: Determination of Off-Odors and Other Volatile Organics In Food Packaging Films By Direct Thermal Analysis-GC-MS
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- Food Science Thermal Desorption Application NotesNote 102: Identification of Contaminants in Powdered Beverages by Direct Extraction Thermal Desorption GC/MS Note 101: Identification of Contaminants in Powdered Foods by Direct Extraction Thermal Desorption GC/MS Note 98: Flavor and Aroma Profiles of Truffle Oils by Thermal Desorption GC/MS Note 93: Detection of Benzene in Carbonated Beverages with Purge & Trap Thermal Desorption GC/MS Note 43: Volatile Organic Composition In Blueberries Note 41: Hydrocarbon Production in Pine by Direct Thermal Extraction Note 35: Volatile Organics Composition of Cranberries Note 33: Changes in Volatile Organic Composition in Milk Over Time Note 31: Volatile Organic Composition in Several Cultivars of Peaches Note 30: Comparison Of Cooking Oils By Direct Thermal Extraction and Purge and Trap GC/MS Note 25: Flavor and Aroma in Natural Bee Honey Note 21: Detection and Identification Of Volatile and Semi-Volatile Organics In Synthetic Polymers Used In Food and Pharmaceutical Packaging Note 18: Determination of Volatile Organic Compounds In Mushrooms Note 17: Identification of Volatile Organics in Wines Over Time Note 14: Identification of Volatiles and Semi-Volatiles In Carbonated Colas Note 12: Identification of the Volatile and Semi-Volatile Organics In Chewing Gums By Direct Thermal Desorption Note 11: Flavor/Fragrance Profiles of Instant and Ground Coffees By Short Path Thermal Desorption Note 9: Methodologies For the Quantification Of Purge and Trap Thermal Desorption and Direct Thermal Desorption Analyses Note 8: Detection of Volatile Organic Compounds In Liquids Utilizing the Short Path Thermal Desorption System Note 6: Direct Thermal Analysis of Plastic Food Wraps Using the Short Path Thermal Desorption System Note 5: Direct Thermal Analysis Using the Short Path Thermal Desorption System Note 4: Direct Analysis of Spices and Coffee Note 1: Determination of Off-Odors and Other Volatile Organics In Food Packaging Films By Direct Thermal Analysis-GC-MS
- Purge & Trap Thermal Desorption Application NotesNote 97: Flavor Profiles of Imported and Domestic Beers by Purge & Trap Thermal Desorption GC/MS Note 93: Detection of Benzene in Carbonated Beverages with Purge & Trap Thermal Desorption GC/MS Note 43: Volatile Organic Composition In Blueberries Note 42: The Influence of Pump Oil Purity on Roughing Pumps Note 38: A New Micro Cryo-Trap For Trapping Of Volatiles At the Front Of a GC Capillary Column Note 35: Volatile Organics Composition of Cranberries Note 34: Selection Of Thermal Desorption and Cryo-Trap Parameters In the Analysis Of Teas Note 33: Changes in Volatile Organic Composition in Milk Over Time Note 32: Selection and Use of Adsorbent Resins for Purge and Trap Thermal Desorption Applications Note 31: Volatile Organic Composition in Several Cultivars of Peaches Note 30: Comparison Of Cooking Oils By Direct Thermal Extraction and Purge and Trap GC/MS Note 26: Volatile Organics Present in Recycled Air Aboard a Commercial Airliner Note 25: Flavor and Aroma in Natural Bee Honey Note 23: Frangrance Qualities in Colognes Note 22: Comparison Of Volatile Compounds In Latex Paints Note 21: Detection and Identification Of Volatile and Semi-Volatile Organics In Synthetic Polymers Used In Food and Pharmaceutical Packaging Note 20: Using Direct Thermal Desorption to Assess the Potential Pool of Styrene and 4-Phenylcyclohexene In Latex-Backed Carpets Note 18: Determination of Volatile Organic Compounds In Mushrooms Note 17: Identification of Volatile Organics in Wines Over Time Note 16: Analysis of Indoor Air and Sources of Indoor Air Contamination by Thermal Desorption Note 14: Identification of Volatiles and Semi-Volatiles In Carbonated Colas Note 8: Detection of Volatile Organic Compounds In Liquids Utilizing the Short Path Thermal Desorption System Note 3: Indoor Air Pollution Note 2: Detection of Arson Accelerants Using Dynamic Headspace with Tenax® Cartridges Thermal Desorption and Cryofocusing
- GC Cryo-Trap Application NotesNote 43: Volatile Organic Composition In Blueberries Note 42: The Influence of Pump Oil Purity on Roughing Pumps Note 41: Hydrocarbon Production in Pine by Direct Thermal Extraction Note 40: Comparison of Septa by Direct Thermal Extraction Note 39: Comparison of Sensitivity Of Headspace GC, Purge and Trap Thermal Desorption and Direct Thermal Extraction Techniques For Volatile Organics Note 38: A New Micro Cryo-Trap For Trapping Of Volatiles At the Front Of a GC Capillary Column Note 37: Volatile Organic Emissions from Automobile Tires Note 36: Identification Of Volatile Organic Compounds In a New Automobile Note 34: Selection Of Thermal Desorption and Cryo-Trap Parameters In the Analysis Of Teas Note 31: Volatile Organic Composition in Several Cultivars of Peaches Note 30: Comparison Of Cooking Oils By Direct Thermal Extraction and Purge and Trap GC/MS Note 29: Analysis Of Volatile Organics In Oil Base Paints By Automated Headspace Sampling and GC Cryo-Focusing Note 28: Analysis Of Volatile Organics In Latex Paints By Automated Headspace Sampling and GC Cryo-Focusing Note 27: Analysis of Volatile Organics In Soils By Automated Headspace GC Note 24: Selection of GC Guard Columns For Use With the GC Cryo-Trap Note 19: A New Programmable Cryo-Cooling/Heating Trap for the Cryo-Focusing of Volatiles and Semi-Volatiles at the Head of GC Capillary Columns
- Application NotesNote 103: EPA Method 325B, Novel Thermal Desorption Instrument Modification to Improve Sensitivity Note 102: Identification of Contaminants in Powdered Beverages by Direct Extraction Thermal Desorption GC/MS Note 101: Identification of Contaminants in Powdered Foods by Direct Extraction Thermal Desorption GC/MS Note 100: Volatile and Semi-Volatile Profile Comparison of Whole Versus Cracked Versus Dry Homogenized Barley Grains by Direct Thermal Extraction Note 99: Volatile and Semi-Volatile Profile Comparison of Whole vs. Dry Homogenized Wheat, Rye and Barley Grains by Direct Thermal Extraction GC/MS Note 98: Flavor and Aroma Profiles of Truffle Oils by Thermal Desorption GC/MS Note 97: Flavor Profiles of Imported and Domestic Beers by Purge & Trap Thermal Desorption GC/MS Note 96: Reducing Warping in Mass Spectrometer Filaments, with SISAlloy® Yttria/Rhenium Filaments Note 95: Detection of Explosives on Clothing Material by Direct and AirSampling Thermal Desorption GC/MS Note 94: Detection of Nepetalactone in the Nepeta Cataria Plant by Thermal Desorption GC/MS Note 93: Detection of Benzene in Carbonated Beverages with Purge & Trap Thermal Desorption GC/MS Note 92: Yttria Coated Mass Spectrometer Filaments Note 91: AutoProbe DEP Probe Tip Temperatures Note 90: An Automated MS Direct Probe for use in an Open Access Environment Note 89: Quantitation of Organics via a Mass Spectrometer Automated Direct Probe Note 88: Analysis of Silicone Contaminants on Electronic Components by Thermal Desorption GC-MS Note 87: Design and Development of an Automated Direct Probe for a Mass Spectrometer Note 86: Simulation of a Unique Cylindrical Quadrupole Mass Analyzer Using SIMION 7.0. Note 85: Replacing an Electron Multiplier in the Agilent (HP) 5973 MSD Note 84: Vacuum Pump Exhaust Filters - Charcoal Exhaust Traps Note 83: Vacuum Pump Exhaust Filters - Oil Mist Eliminators Note 82: Vacuum Pump Exhaust Filters Note 81: Rapid Bacterial Chemotaxonomy By DirectProbe/MSD Note 80: Design, Development and Testing of a Microprocessor ControlledAutomated Short Path Thermal Desorption Apparatus Note 79: Volatile Organic Compounds From Electron Beam Cured and Partially Electron Beam Cured Packaging Using Automated Short Path Thermal Desorption Note 78: A New Solution to Eliminate MS Down-Time With No-Tool-Changing of Analytical GC Columns Note 77: The Determination of Volatile Organic Compounds in VacuumSystem Components Note 76: Determination of the Sensitivity of a CRIMS System Note 75: An Apparatus for Sampling Volatile Organics From LivePlant Material Using Short Path Thermal Desorption Note 74: Examination of Source Design in Electrospray-TOF Using SIMION 3D Note 73: The Analysis of Perfumes and their Effect on Indoor Air Pollution Note 72: 1998 Version of the NIST/EPA/NIH Mass Spectral Library, NIST98 Note 71: Flavor Profile Determination of Rice Samples Using Shor tPath Thermal Desorption GC Methods Note 70: Application of SIMION 6.0 To a Study of the Finkelstein Ion Source: Part II Note 69: Application of SIMION 6.0 To a Study of the Finkelstein Ion Source: Part 1 Note 68: Use of a PC Plug-In UV-Vis Spectrometer To Monitor the Plasma Conditions In GC-CRIMS Note 67: Using Chemical Reaction Interface Mass Spectrometry (CRIMS) To Monitor Bacterial Transport In In Situ Bioremediation Note 66: Probe Tip Design For the Optimization of Direct Insertion Probe Performance Note 65: Determination of Ethylene by Adsorbent Trapping and Thermal Desorption - Gas Chromatography Note 64: Comparison of Various GC/MS Techniques For the Analysis of Black Pepper (Piper Nigrum) Note 63: Determination of Volatile and Semi-Volatile Organics in Printer Toners Using Thermal Desorption GC Techniques Note 62: Analysis of Polymer Samples Using a Direct Insertion Probe and EI Ionization Note 61: Analysis of Sugars Via a New DEP Probe Tip For Use With theDirect Probe On the HP5973 MSD Note 60: Programmable Temperature Ramping of Samples Analyzed ViaDirect Thermal Extraction GC/MS Note 59: Computer Modeling of a TOF Reflectron With Gridless Reflector Using SIMION 3D Note 58: Direct Probe Analysis and Identification of Multicomponent Pharmaceutical Samples via Electron Impact MS Note 57: Aroma Profiles of Lavandula species Note 56: Mass Spec Maintenance & Cleaning Utilizing Micro-Mesh® Abrasive Sheets Note 55: Seasonal Variation in Flower Volatiles Note 54: Identification of Volatile Organic Compounds in Office Products Note 53: SIMION 3D v6.0 Ion Optics Simulation Software Note 52: Computer Modeling of Ion Optics in Time-of-Flight mass Spectrometry Using SIMION 3D Note 51: Development and Characterization of a New Chemical Reaction Interface for the Detection of Nonradioisotopically Labeled Analytes Using Mass Spectrometry (CRIMS) Note 50: The Analysis of Multiple Component Drug Samples Using a Direct Probe Interfaced to the HP 5973 MSD Note 49: Analysis of Cocaine Utilizing a New Direct Insertion Probe on a Hewlett Packard 5973 MSD Note 48: Demonstration of Sensitivity Levels For the Detection of Caffeine Using a New Direct Probe and Inlet for the HP 5973 MSD Note 47: The Application Of SIMION 6.0 To Problems In Time-of-Flight Mass Spectrometry Note 46: Delayed Extraction and Laser Desorption: Time-lag Focusing and Beyond Note 45: Application of SIMION 6.0 to Filament Design for Mass Spectrometer Ionization Sources Note 44: The Design Of a New Direct Probe Inlet For a Mass Spectrometer Note 43: Volatile Organic Composition In Blueberries Note 42: The Influence of Pump Oil Purity on Roughing Pumps Note 41: Hydrocarbon Production in Pine by Direct Thermal Extraction Note 40: Comparison of Septa by Direct Thermal Extraction Note 39: Comparison of Sensitivity Of Headspace GC, Purge and Trap Thermal Desorption and Direct Thermal Extraction Techniques For Volatile Organics Note 38: A New Micro Cryo-Trap For Trapping Of Volatiles At the Front Of a GC Capillary Column Note 37: Volatile Organic Emissions from Automobile Tires Note 36: Identification Of Volatile Organic Compounds In a New Automobile Note 35: Volatile Organics Composition of Cranberries Note 34: Selection Of Thermal Desorption and Cryo-Trap Parameters In the Analysis Of Teas Note 33: Changes in Volatile Organic Composition in Milk Over Time Note 32: Selection and Use of Adsorbent Resins for Purge and Trap Thermal Desorption Applications Note 31: Volatile Organic Composition in Several Cultivars of Peaches Note 30: Comparison Of Cooking Oils By Direct Thermal Extraction and Purge and Trap GC/MS Note 29: Analysis Of Volatile Organics In Oil Base Paints By Automated Headspace Sampling and GC Cryo-Focusing Note 28: Analysis Of Volatile Organics In Latex Paints By Automated Headspace Sampling and GC Cryo-Focusing Note 27: Analysis of Volatile Organics In Soils By Automated Headspace GC Note 26: Volatile Organics Present in Recycled Air Aboard a Commercial Airliner Note 25: Flavor and Aroma in Natural Bee Honey Note 24: Selection of GC Guard Columns For Use With the GC Cryo-Trap Note 23: Frangrance Qualities in Colognes Note 22: Comparison Of Volatile Compounds In Latex Paints Note 21: Detection and Identification Of Volatile and Semi-Volatile Organics In Synthetic Polymers Used In Food and Pharmaceutical Packaging Note 20: Using Direct Thermal Desorption to Assess the Potential Pool of Styrene and 4-Phenylcyclohexene In Latex-Backed Carpets Note 19: A New Programmable Cryo-Cooling/Heating Trap for the Cryo-Focusing of Volatiles and Semi-Volatiles at the Head of GC Capillary Columns Note 18: Determination of Volatile Organic Compounds In Mushrooms Note 17: Identification of Volatile Organics in Wines Over Time Note 16: Analysis of Indoor Air and Sources of Indoor Air Contamination by Thermal Desorption Note 14: Identification of Volatiles and Semi-Volatiles In Carbonated Colas Note 13: Identification and Quantification of Semi-Volatiles In Soil Using Direct Thermal Desorption Note 12: Identification of the Volatile and Semi-Volatile Organics In Chewing Gums By Direct Thermal Desorption Note 11: Flavor/Fragrance Profiles of Instant and Ground Coffees By Short Path Thermal Desorption Note 10: Quantification of Naphthalene In a Contaminated Pharmaceutical Product By Short Path Thermal Desorption Note 9: Methodologies For the Quantification Of Purge and Trap Thermal Desorption and Direct Thermal Desorption Analyses Note 8: Detection of Volatile Organic Compounds In Liquids Utilizing the Short Path Thermal Desorption System Note 7: Chemical Residue Analysis of Pharmaceuticals Using The Short Path Thermal Desorption System Note 6: Direct Thermal Analysis of Plastic Food Wraps Using the Short Path Thermal Desorption System Note 5: Direct Thermal Analysis Using the Short Path Thermal Desorption System Note 4: Direct Analysis of Spices and Coffee Note 3: Indoor Air Pollution Note 2: Detection of Arson Accelerants Using Dynamic Headspace with Tenax® Cartridges Thermal Desorption and Cryofocusing Note 1: Determination of Off-Odors and Other Volatile Organics In Food Packaging Films By Direct Thermal Analysis-GC-MS Tech No. "A" Note 14: Elimination of "Memory" Peaks in Thermal Desorption Improving Sensitivity in the H.P. 5971 MSD and Other Mass Spectrometers - Part I of II Improving Sensitivity in the H.P. 5971 MSD and Other Mass Spectrometers- Part II of II Adsorbent Resins Guide Development and Field Tests of an Automated Pyrolysis Insert for Gas Chromatography. Hydrocarbon Production in Pine by Direct Thermal Extraction A New Micro Cryo-Trap for the Trapping of Volatiles at the Front of a GC Capillary (019P) - Comparison of Septa by Direct Thermal Extraction Volatile Organic Composition in Blueberry Identification of Volatile Organic Compounds in Office Products Detection and Indentification of Volatiles in Oil Base Paintsby Headspace GC with On Column Cryo-Trapping Evaluation of Septa Using a Direct Thermal Extraction Technique INFLUENCE OF STORAGE ON BLUEBERRY VOLATILES Selection of Thermal Desorption and Cryo-Trap Parameters in the Analysis of Teas Redesign and Performance of a Diffusion Based Solvent Removal Interface for LC/MS The Design of a New Direct Probe Inlet for a Mass Spectrometer Analytes Using Mass Spectrometry (CRIMS) Application of SIMION 6.0 to Filament Design for Mass Spectrometer Ionization Sources A Student Guide for SIMION Modeling Software Application of SIMION 6.0 to Problems in Time-of-flight Mass Spectrometry Comparison of Sensitivity of Headspace GC, Purge and TrapThermal Desorption and Direct Thermal Extraction Techniques forVolatile Organics The Influence of Pump Oil Purity on Roughing Pumps Analysis of Motor Oils Using Thermal Desorption-Gas Chromatography-Mass Spectrometry IDENTIFICATION OF VOLATILE ORGANIC COMPOUNDS IN PAPER PRODUCTS Computer Modeling of Ion Optics in Time-of-Flight mass Spectrometry using SIMION 3D Seasonal Variation in Flower Volatiles Development of and Automated Microprocessor Controlled Gas chromatograph Fraction Collector / Olfactometer Delayed Extraction and Laser Desorption: Time-lag Focusing and Beyond A New Micro Cryo-Trap for the Trapping of Volatiles at the Front of a GC Column Design of a Microprocessor Controlled Short Path Thermal Desorption Autosampler Computer Modeling of Ion Optics in Time-of-Flight Mass Spectrometry Using SIMION 3D Thermal Desorption Instrumentation for Characterization of Odors and Flavors
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- Note 43: Volatile Organic Composition In Blueberries (This Page)
1999
INTRODUCTION
Volatile and semi-volatile organic compounds present both in the sample matrix and in the headspace aroma exhibit a tremendous influence on the flavor/fragrance qualities of blueberries. There is a concern in the blueberry industry as to what constitutes the optimum conditions for harvesting blueberries so as to provide a consistent quality and flavor of blueberry to the consumer. Several investigations have reported that the concentrations of volatile constituents increased with the maturation of blueberries. The major volatiles of blueberries appear to be useful indices for determining maturity, and recently, the determination of flavor precursors and intermediates have become the target of flavor studies. To date, headspace GC analysis, cryofocusing techniques, and high resolution GC have been used for the analysis of blueberry volatiles from promising cultivars under development. However, static headspace techniques are limited in their detection and identification of many organic volatiles and especially semi-volatile organics. Other techniques are needed to profile a wider range of volatile and semi-volatile organics in blueberries and to identify the flavors, fragrances, off-flavors, off-odors and potential contaminants that may be present. The purpose of this investigation is to develop an analytical technique that could detect and identify a wide range of volatile and semi-volatile organic compounds in blueberries. For this study, volatile organic compounds are purged from blueberry samples followed by trapping on Tenax® TA adsorbent resin using a dynamic purge and trap technique (P&T), and then chromatographed on two different columns to compare and quantitate the volatile organics present. The adsorbent traps are subsequently analyzed by thermal desorption-gas chromatography-mass spectrometry (TD-GC-MS). The P&T technique permits the analysis of a wider range of both volatile and semi-volatile organic compounds and is more sensitive as compared to the static headspace technique.
Instrumentation
Figure 1 - Purge and Trap Of Blueberries
Samples were collected using a Scientific Instrument Services Purge and Trap System. This apparatus (Fig. 1) consists of a sparge gas inlet connected to a stainless steel purging needle that is inserted through an adaptor fitting into the blueberry liquid at the bottom of the 10 ml purge and trap test tube. A dry purge gas inlet is located at a right angle to the sparge gas inlet at the top of the apparatus. The purpose of the dry purge is to reduce the water vapor condensation on the adsorbent trap. Opposite the dry purge inlet is the connector for the desorption tube. To this fitting a glass-lined stainless steel (GLT) desorption tube containing the adsorbent resin is attached for the trapping of the purged volatiles.
Samples were desorbed into the GC injection port using the SIS Model TD-3 Short Path Thermal Desorption System. The desorption tube blocks were set to 220 degrees C and the desorption system flow rate was set to 12 ml/min. The desorption system timer was set to 5.0 minutes for the desorption system cycle.
For both systems described below, the head of each capillary column was maintained at -70 degrees C using an S.I.S. Cryotrap model 951 during the desorption and extraction process and then ballistically heated to 200 degrees C to release the volatiles after which the GC oven was temperature programmed from 35 degrees C (hold for 5 minutes) to 80 degrees C at 10 degrees C/min, then to 200 degrees C at 4 Degrees C/min and finally to 260 degrees C at a rate of 10 degrees C/min.
System A - The experiments were conducted using a Scientific Instrument Services model TD-3 Short Path Thermal Desorption System accessory connected to the injection port of a HP 5890 Series II GC interfaced to an HP 5971 Mass Selective Detector (MSD). The HP 5890 Series II GC contained a short 0.5 meter by 0.53 mm diameter fused silica precolumn attached to the injection port end of a 30 meter x 0.25 mm i.d. DB-5MS J&W capillary column containing a 0.25 micron film thickness. The GC injection port was set to 260 degrees C and a 10:1 split was used. The mass spectrometers were operated in the electron impact mode (EI) and scanned from 35 to 550 daltons during the GC run for the total ion chromatogram. The HP Chem Station software with the Wiley NBS library was used to analyze the eluted volatiles and identify each compound.
System B. The experiments were conducted using a Scientific Instrument Services model TD-3 Short Path Thermal Desorption System accessory connected to the injection port of a HP 5890 Series II GC with electronic pressure control interfaced to an HP Engine Mass Spectrometer. A SGE (Scientific Glass Engineering, Austin Texas) BPX35 capillary column, 60 meters long by 0.22 mm i.d. x 0.25 micron film thickness was used in the GC oven. The GC injection port was set to 220 degrees C. The mass spectrometers were operated in the electron impact mode (EI) and scanned from 35 to 550 daltons during the GC run for the total ion chromatogram. The HP Chem Station software with the Wiley NBS library was used to analyze the eluted volatiles and identify each compound.
Experimental
Three varieties of blueberry (Jersey lowbush, Bluecrop lowbush & Rancocas lowbush) were analyzed by a dynamic P&T technique and chromatographed on both a 30 meter DB-5MS capillary column and a 60 meter BPX35 capillary column to compare and quantitate the volatile organics present. For quantification, a deuterated cymene internal standard was spiked into the adsorbent traps after the volatiles from the blueberries had been adsorbed onto the adsorbent resin traps. No correction for extraction efficiency of recovery is achieved using this technique; however, it functions as a useful means of quantifying the levels of components present on the adsorbent traps.
Approximately 100 grams of blueberry were homogenized for 3 minutes using a Waring® commercial blender. Sample sizes of 10-11 grams of this blueberry homogenate were then transferred into a 10 ml purge and trap test tube and heated to 60 degrees C in a water bath. Samples were then purged for 45 minutes with high purity helium at 20 ml/min (900 ml total) with an additional 25 ml/min dry purge using the S.I.S. Purge and Trap system. The volatile analytes were purged onto a preconditioned 4.0 mm i.d. glass-lined stainless steel desorption tube packed with 200 mg of Tenax TA. The samples were subsequently spiked with 100 ng of d-cymene internal standard by injecting 1 ul of a 100 ng/ul stock solution of a d-14 cymene in methanol by syringe injection into the Tenax matrix, and then purged for an additional 5 minutes with helium at 50 ml/min to remove the methanol. The desorption tube with sample and internal standard was then attached to the Short Path Thermal Desorption System and a syringe needle attached. The desorption tube was injected into the GC injection port and the volatiles desorbed into the GC at desorption block temperatures of 220 degrees C for 5 minutes and a flow rate of 12 ml/min. The volatiles were cryo-focused at -70 degrees C at the front of the GC column during the desorption process and then released onto the GC column by rapidly heating the cryo-trap to 220 degrees C. The GC column temperature program was initiated and the eluted analytes detected and identified via the mass spectrometer.
Results and Discussion
Fig. 2 - Jersey Lowbush analyzed On J&W DB-5MS Column
Fig. 3 - Rancocas Lowbush Analyzed On J&W DB-5MS Column
Fig. 4 - Bluecrop Lowbush Analyzed On J&W DB-5MS Column
Three varieties of blueberry were analyzed to identify, compare and quantify the volatile organics present using both the SGE BPX35 and the J&W DB-5MS capillary columns, and from the data, determine the most sensitive method of analysis. Over 100 volatile organics were identified in the blueberries studied. The blueberries studied produced 50 or more volatile organics which were identified in addition to many more that were either too weak or in which a good NBS library match was not achievable. The blueberries possessed numerous mono- and sesquiterpenoid compounds as well as numerous straight and branched chain hydrocarbons, aldehydes, alcohols, ketones and esters (Figs. 2-7). Although they possess many common compounds, each blueberry variety had its own distinct fingerprint chromatograph.
The SGE BPX35 capillary column provided better separation and resolution for the three blueberry samples analyzed (Figs. 5-7) than the J&W DB-5MS (Figs. 2-4) capillary column for this application . Due to the slightly higher polarity of the BPX35 column the more polar alcohols, aldehydes and acids produced highly resolved GC peaks which were easily detected and identified. Many of these polar compounds were not resolved and identified with the non polar DB5-MS column.
The blueberries were found to contain the flavor compound benzaldehyde which has an almond-like odor and the aliphatic compounds hexanal, heptanal and nonanal (Figs. 2-7). It has been assumed that unsaturated fatty acids, primarily linoleic and linolenic acids, are the precursors of these aliphatic compounds and may contribute to the development of rancid flavor. The presence of the branched aldehydes 3-methyl-butyraldehyde and 2-methyl-butyraldehyde in each of the blueberries (Figs. 2-4) contribute to the fruity flavor notes as well as reflect the microbial quality of the blueberry. The predominant terpenes included 1,8-cineole and linalool with trace amounts of cymene, limonene, 1-terpineol, trans-caryophyllene (Figs. 2-4) and 3-carene (Figs. 5-7). Citral which has a strong lemon odor was also identified in the Rancocas and Bluecrop varieties (Figs. 3&4).
Fig. 5 - Jersey Lowbush Analyzed On SGE BPX-35 Column
Fig. 6 - Rancocas Lowbush Analyzed On SGE BPX-35 Column
Fig. 7 - Bluecrop Lowbush Analyzed On SGE BPX-35 Column
The major esters found in the blueberry fruit included ethyl acetate and 3-isopropyl-buyrate. It is generally considered that esters primarily contribute to the fruity and floral notes. SGE's BPX35 capillary column (Figs. 5-7) provided additional information to the volatile organic composition of blueberries with the identification of the alcohols 1-butanol, 1-pentanol, 1-hexanol, 2-ethyl-1-hexanol, 1-heptanol, 1-octanol and 1-nonanol which were not detected with J&W's DB-5MS column. The production of these alcohols may be characteristic of the fruit during maturation. Linalool oxide, an isomer of lilac alcohol, and tetrahydro-furfuryl-(2)-alcohol were identified in the Jersey and Rancocas varieties (Figs. 2&3) while the higher molecular weight compound decadienal was found in each of the varieties (Figs. 2-4). This compound contributes to the flavor and aroma of the blueberries and is the result of fatty acid decomposition.
The Jersey and Bluecrop varieties exhibited several furan derivatives (Figs. 5&7) which reflect the microbiological purity and storage conditions of the blueberries. The antioxidant butylated hydroxytoluene was detected in each of the blueberries and is probably derived from the packaging material during storage. Other middle-chain aliphatic alcohols, aldehyde and ketone derivatives were also found in each of the varieties of blueberry.
Conclusion
The Short Path Thermal Desorption System used in conjunction with a dynamic purge and trap technique along with the appropriate capillary column permits the identification and quantification of trace levels of volatile organics in blueberries. The method described above permits both the qualitative and quantitative analysis of blueberries to identify the volatile organics, flavoring compounds, contaminants and off-odor compounds present. This technique has proven effective in detecting and identifying a larger number of organic compounds at concentrations lower than was previously obtainable via other analysis techniques, such as static headspace GC analysis. It also represents a tremendous improvement over the time-consuming solvent extraction techniques normally used in the laboratory. This technique can be easily incorporated into a troubleshooting technique to detect problems in a wide variety of commercial food products, to compare various competing manufacturers products, as well as a quality control program.
In addition the SGE BPX-35 mid polarity capillary column was found to be superior for the analysis of blueberries and other food products which contain numerous aldehydes and alcohols in the volatile flavor profile.