- ▶
- Heaters/Source
- ▶
- Agilent Heaters and SensorsMass Spectrometry, Scientific Supplies & ManufacturingScientific Instrument Services 5973 Source Heater Tamper Resistant Allen Wrench 5973/5975 Quad Sensor 5985 Source Heater Assembly Agilent Interface Heater Assembly 5971 Interface Heater
- ▶
- LiteratureApplication Notes Adsorbent Resins Guide Mass Spec Tips SDS Sheets FAQ MS Calibration Compound Spectra Manuals MS Links/Labs/ Organizations MS Online Tools Flyers on Products/Services Scientific Supplies Catalog About Us NextAdvance Bullet Blender® Homogenizer Protocols Micro-Mesh® Literature Instrumentation Literature Agilent GC/MS Literature SIS News / E-Mail Newsletter NIST MS Database - Update Notifications
- ▶
- Mass Spec Tips1 - Freon for use in Mass Spectrometer Leak Checking 2 - Mass Spectrometer Probe Cooling 3 - Sample Vials for Direct Probes 4 - Selection of Vacuum Pump Oils for Lowest Mass Spec Background 5 - Determination Linkages in Biomolecules of Disulfide 6 - Transfer of H.P. ChemStation MS and GC Chromatograms from PC to MAC Computer 7 - Repairing Clogged Thermospray Probes 8 - Tuning a Finnigan 5100 to Meet BFB or DFTPP Criteria 9 - H.P. 5971 Transfer Line Tip for Direct Introduction of Capillary Column 10 - Troubleshooting Finnigan 5100 GC/MS Systems 11 - Leak Checking Mass Spectrometers 12 - Elimination of Memory Peaks and GC Background Noise 13 - SuperIncos Mapped Software Print Buffer Lockup 14 - Reduction of Peak Tailing 15 - Electron Multiplier Sensitivity 16 - INCOS Procedure for Calibrating on the Finnigan 4500 17 - Extending Electron Multiplier Life 18 - What techniques or methods do you use to determine if the electron multiplier 19- What techniques or methods do you use to detect vacuum leaks in your mass spectrometer 20 - Extending Lenear Range of the Mass Spec Article - Improving Sensitivity in the HP 5971 Mass Spectrometer - Part 1 and Part 2
- ▶
- 5 - Determination Linkages in Biomolecules of Disulfide (This Page)
Mass Spec Tips is a collection of tips relating to the operation
and service of mass spectrometers. Some of these Tips have been
reported in our newsletter, "The Mass Spec Source."
If you have a question relating to the operation or service of
mass spectrometers or would like to add your comments to the below
tip and would like to have it included in this forum, please contact us.
Input to this database of MS Tips is needed from our readers
to complete this section.
Author: Anonymous
Affiliation: Pharmaceutical Industry
A fast and easy way to detect the presence of disulfide linkages in peptides during static FAB or LISIMS analysis is to dissolve the analyte in a sulfur containing matrix (i.e., thioglycerol, magic bullet, etc.) and add a trace amount of dilute ammonium hydroxide. We have found that this mixture causes immediate reduction of disulfide linkages (converting them to free-SH groups) with the expected 2 dalton mass increase per disulfide group.