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- Easy sample tube removal since the tube protrudes above the bottom section of the pressure injection cell.
- O-ring seal at interface ensures no leakage.
- Rated for 2500 psi (17,000kPa), ideal for consistent results with bottled gas.
- Available in 8500 psi (56,000 kPa) version
- Includes a 3-way valve for easy switching between pressurization and release of pressure
- Three bolt system secures the cell, allows easy addition and removal of tubes / vials
- Holds 0.5 to 2 mL microcentrifuge/Eppendorf tubes and 12 x 32 mm (glass) vials.
- Includes operator's manual; 10 reusable ferrules for typical (380 µm outside diameter) capillaries (other ferrules are available); and 1.5 mL of Kasil and 0.5 mL of formamide and a cleaving tool for making your own frits.
- Available with integrated magnetic stirplate.
- Warranty: 2 years parts and labor
- 30 day money back guarantee
The Pressure Injection Cell, sometimes called a "bomb loader" enables controlled dispensing of small-volume liquid samples. Using high pressure, the pressur injection cell has proved invaluable for two applications: densely packing nanobore capillary columns with solid-phase particles; and precisely infusing microlitr samples directly from microcentrifuge tubes into mass spectrometers without additional transfers, wasted sample, or contact with metallic surfaces which adsorb some negatively charged molecules such as peptides with phospho or carboxy groups.
The Pressure Injection Cell holds 1 mL and 2 mL micro-centrifuge tubes as well as 12 x 32 mm glass vials in its central chamber (shown at right). The assembly can be used on a typical magnetic stirrer, e.g. to keep particles in suspension. The cell needs to be connected to a bottle/tank of compressed gas, such as Helium or Nitrogen. Packing capillary columns requires several hundred to about 1000 psi or sometimes more, and mass specrometer injections typically require a few hundred psi. this unit is rated for a 2500 psi. 8500 psi units are available
Pack Your Own LC/MS Capillary Columns
Why pay hundreds of dollars per packed capillary column for LC/MS? You can pack the columns, of various internal diameters, yourself. The Pressure Injection Cell comes with instructions on how to pack columns and is available as a stand alone unit (with 9 extra ferrules), or as a complete kit with ferrules, 1/8" stainless steel tubing, a spool of capillary, a cleaving tool, and a frit assembly, or a paritial kit.
The Pressure Injection Cell is made with the finest methods and components, thereby enabling you to acquire very accurate data. The high quality stainless steel fittings and valve are made by Swagelok. The body and cap of the pressure chamber are nickel coated. Special features not found elsewhere include a recess in the cap to allow easy access to the sample tube and a hexagonal shape to help align the cap to the body. The Pressure Injection Cell's simple, rugged design and the use of top components ensure years of trouble free use and quality performance./p>
Load Samples into Mass Spectrometer
With the Pressure Injection Cell, load your sample into a mass spectrometer directly from a microcentrifuge or Eppenorf tube and avoid transfer loss. There is no contact with metallic surfaces which would adsorb negatively charged molecules.
The Pressure Injection Cell comes with a 30 day money back guarantee and a 2 year warranty
Operationally, the Pressure Injection Cell is analogous to a straw in a juice box. As you apply pressure in the box, the juice will be forced out through the straw. Likewise, the Pressure Injection Cell is connected to pressurized gas, typically in a tank. A 0.5 to 2 mL tube containing the sample liquid is placed in the base of the Pressure Injection Cell. A capillary is then placed through the ferrule in the cap and down into the sample tube. When packing a capillary, a frit assembly is placed at the distal end of the capillary to prevent particles from exiting and the entire Pressure Injection Cell is typically placed on a stir plat. By regulating the gas pressure, you can adjust the flow rate of the sample into the capillary.
On the left is a LC/MS chromatogram of a peptide mixture prepared from a tryptic digested gel band, separated by a 5 µm C18 resin packed column (100 µm inside diameter by 10 cm long capillary). The Pressure Injection Cell ws used to pack the LC/MS capillary column and to inject the sample into the mass spectrometer. Courtesy of Dr Qishan Lin.
Schematic of System with Pressure Injection Cell
|Body Material||Acrylic||Stainless Steel||Stainless Steel||Stainless Steel||Stainless Steel|
PC1000 Transparent Pressure Injection Cell
Look inside while packing your column!
The PC1000 has a trasparent body, so you can see the level of packing suspension in the vial. Rated for 1000 psi-sufficient for most column packing applications. Orders include a frit kit, a hex wrench, 10 ferrules for standard 400 micrometer outside diameter capillary, and an owners manual
PC77 Pressure Injection Cell
The PC77 Pressure Injection Cell is rated for 2500 psi (17,000 kPa). Includes a three-way valve for convenience. Includes a hex wrench, 10 ferrules for typical (~363 µm external diameter) capillaries, a frit kit, and operator manual
PC77-MAG Pressure Injection Cell
The PC77-MAG Pressure Injection Cell with integrated magnetic stir plate for keeping particles in suspension when packing capillaries. Also includes a 5 x 2 mm stirbar, a hex wrench, 10 ferrules for typical (~363 µm external diameter) capillaries, a frit kit, and operator manual
PC8500 Pressure Injection Cell
The PC8500 Pressure Injection Cell. High pressure version of PC77, rated for 8500 psi (58,000 kPa). Includes a hex wrench, 10 ferrules for typical (~363 µm external diameter) capillaries, a frit kit, and operator manual
PC8500-MAG Pressure Injection Cell
The PC8500-MAG Pressure Injection Cell similar to PC77-MAG, except with a 8500 psi rating. this model has an integrated magnetic stir plate for keeping particles in suspension when packing capillaries Also includes a 5 x 2 mm stirbar, a hex wrench, 10 ferrules for typical (~363 µm external diameter) capillaries, a frit kit, and operator manual
Does the Pressure Injection Cell require electrical power?
No. The liquid sample is forced through the capillary using pressurized gas typically supplied by a tank Models with an integrated magnetic stirplate require electricity. They use a small power supply that plugs into a wall outlet. We supply the correct plug for your country.
Which gases can it use?
The choice of gas is not critical. Most customers use inert gases such as helium, nitrogen or argon. Dry air is fine too - if you purchase an adapter, NIP-AIR, to interface the tank with the pressure regulator.
Which adapters do I need?
If you're operating the pressure injection cell with inert gas in the United States or other country using English parts and CGA fittings (580 for inert gases), you do not need any adapters.
If you will use metric stainless steel tubing, you will need an adapter, ADPT-3mm1/8 to mate the 3 mm tubing with the 1/8 inch fitting on the pressure injection cell. This can be factory installed or installed in the field.
If you will use 1/8" stainless tubing with a regulator with metric or ISO fittings, you will need the adapter, ADPT-ISOto1/8.
How much pressure is required?
Loading samples into a capillary for mass spectroscopy typically requires 100 to 400 psi. Packing capillary columns typically requires 500 to 1000 psi.
Do I need a special pressure regulator?
Most pressure regulators for gas cylinders have a maximum working pressure that is too low for packing capillary columns. We sell a higher pressure regulator, model HPREG, that has a working pressure up to 1500 psi, which is ideal for packing standard length capillary columns.
How can you determine the approximate flow rate through a capillary?
If the solution is flowing only through an otherwise empty capillary tube, the flow rate is straightforward to calculate. However, a frit or a packed capillary typically causes much more flow resistance, so it is best to measure the flow rate with a test solution.
To measure the flow rate, run a test solution through a sample capillary and at the outlet place a calibrated micropipette. The solution should wick up the micropipette on its own so you don't need any special fittings to seal them together. For example, Drummond and other brands of micropipettes, available from most laboratory product suppliers, come in 1, 2, 3, 4, 5, 10, 20, ... &mico;l volumes. Using a stopwatch, measure how long it takes from when the solution starts to exit the capillary and begins filling the micropipette until the micropipette is filled.
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