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MS TIP No. 5 - Determination of Disulfide Linkages in Biomolecules

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Author: Anonymous

Affiliation: Pharmaceutical Industry

A fast and easy way to detect the presence of disulfide linkages in peptides during static FAB or LISIMS analysis is to dissolve the analyte in a sulfur containing matrix (i.e., thioglycerol, magic bullet, etc.) and add a trace amount of dilute ammonium hydroxide. We have found that this mixture causes immediate reduction of disulfide linkages (converting them to free-SH groups) with the expected 2 dalton mass increase per disulfide group.