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- Mass Spec Tips1 - Freon for use in Mass Spectrometer Leak Checking 2 - Mass Spectrometer Probe Cooling 3 - Sample Vials for Direct Probes 4 - Selection of Vacuum Pump Oils for Lowest Mass Spec Background 5 - Determination Linkages in Biomolecules of Disulfide 6 - Transfer of H.P. ChemStation MS and GC Chromatograms from PC to MAC Computer 7 - Repairing Clogged Thermospray Probes 8 - Tuning a Finnigan 5100 to Meet BFB or DFTPP Criteria 9 - H.P. 5971 Transfer Line Tip for Direct Introduction of Capillary Column 10 - Troubleshooting Finnigan 5100 GC/MS Systems 11 - Leak Checking Mass Spectrometers 12 - Elimination of Memory Peaks and GC Background Noise 13 - SuperIncos Mapped Software Print Buffer Lockup 14 - Reduction of Peak Tailing 15 - Electron Multiplier Sensitivity 16 - INCOS Procedure for Calibrating on the Finnigan 4500 17 - Extending Electron Multiplier Life 18 - What techniques or methods do you use to determine if the electron multiplier 19- What techniques or methods do you use to detect vacuum leaks in your mass spectrometer 20 - Extending Lenear Range of the Mass Spec Article - Improving Sensitivity in the HP 5971 Mass Spectrometer - Part 1 and Part 2
- 5 - Determination Linkages in Biomolecules of Disulfide (This Page)
Affiliation: Pharmaceutical Industry
A fast and easy way to detect the presence of disulfide linkages in peptides during static FAB or LISIMS analysis is to dissolve the analyte in a sulfur containing matrix (i.e., thioglycerol, magic bullet, etc.) and add a trace amount of dilute ammonium hydroxide. We have found that this mixture causes immediate reduction of disulfide linkages (converting them to free-SH groups) with the expected 2 dalton mass increase per disulfide group.