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The Pressure Injection Cell, sometimes called a "bomb loader" enables controlled dispensing of small-volume liquid samples. Using high pressure, the pressur injection cell has proved invaluable for two applications: densely packing nanobore capillary columns with solid-phase particles; and precisely infusing microlitr samples directly from microcentrifuge tubes into mass spectrometers without additional transfers, wasted sample, or contact with metallic surfaces which adsorb some negatively charged molecules such as peptides with phospho or carboxy groups.
The Pressure Injection Cell holds 1 mL and 2 mL micro-centrifuge tubes as well as 12 x 32 mm glass vials in its central chamber (shown at right). The assembly can be used on a typical magnetic stirrer, e.g. to keep particles in suspension. The cell needs to be connected to a bottle/tank of compressed gas, such as Helium or Nitrogen. Packing capillary columns requires several hundred to about 1000 psi or sometimes more, and mass specrometer injections typically require a few hundred psi. this unit is rated for a 2500 psi. 8500 psi units are available
Why pay hundreds of dollars per packed capillary column for LC/MS? You can pack the columns, of various internal diameters, yourself. The Pressure Injection Cell comes with instructions on how to pack columns and is available as a stand alone unit (with 9 extra ferrules), or as a complete kit with ferrules, 1/8" stainless steel tubing, a spool of capillary, a cleaving tool, and a frit assembly, or a paritial kit.
The Pressure Injection Cell is made with the finest methods and components, thereby enabling you to acquire very accurate data. The high quality stainless steel fittings and valve are made by Swagelok. The body and cap of the pressure chamber are nickel coated. Special features not found elsewhere include a recess in the cap to allow easy access to the sample tube and a hexagonal shape to help align the cap to the body. The Pressure Injection Cell's simple, rugged design and the use of top components ensure years of trouble free use and quality performance./p>
With the Pressure Injection Cell, load your sample into a mass spectrometer directly from a microcentrifuge or Eppenorf tube and avoid transfer loss. There is no contact with metallic surfaces which would adsorb negatively charged molecules.
The Pressure Injection Cell comes with a 30 day money back guarantee and a 2 year warranty
Operationally, the Pressure Injection Cell is analogous to a straw in a juice box. As you apply pressure in the box, the juice will be forced out through the straw. Likewise, the Pressure Injection Cell is connected to pressurized gas, typically in a tank. A 0.5 to 2 mL tube containing the sample liquid is placed in the base of the Pressure Injection Cell. A capillary is then placed through the ferrule in the cap and down into the sample tube. When packing a capillary, a frit assembly is placed at the distal end of the capillary to prevent particles from exiting and the entire Pressure Injection Cell is typically placed on a stir plat. By regulating the gas pressure, you can adjust the flow rate of the sample into the capillary.
On the left is a LC/MS chromatogram of a peptide mixture prepared from a tryptic digested gel band, separated by a 5µm C18 resin packed column (100 µm inside diameter by 10 cm long capillary). The Pressure Injection Cell ws used to pack the LC/MS capillary column and to inject the sample into the mass spectrometer. Courtesy of Dr Qishan Lin.
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Yttria coated filament at start
Yttria coated filament after 16,000 cycles