Pressure Injection Cell

Pressure Injection Cell

Get Better LC/MS Results and Save Money too!

The Pressure Injection Cell, sometimes called a "bomb loader" enables controlled dispensing of small-volume liquid samples. Using high pressure, the pressur injection cell has proved invaluable for two applications: densely packing nanobore capillary columns with solid-phase particles; and precisely infusing microlitr samples directly from microcentrifuge tubes into mass spectrometers without additional transfers, wasted sample, or contact with metallic surfaces which adsorb some negatively charged molecules such as peptides with phospho or carboxy groups.

The Pressure Injection Cell holds 1 mL and 2 mL micro-centrifuge tubes as well as 12 x 32 mm glass vials in its central chamber (shown at right). The assembly can be used on a typical magnetic stirrer, e.g. to keep particles in suspension. The cell needs to be connected to a bottle/tank of compressed gas, such as Helium or Nitrogen. Packing capillary columns requires several hundred to about 1000 psi or sometimes more, and mass specrometer injections typically require a few hundred psi. this unit is rated for a 2500 psi. 8500 psi units are available

Pack Your Own LC/MS Capillary Columns

Why pay hundreds of dollars per packed capillary column for LC/MS? You can pack the columns, of various internal diameters, yourself. The Pressure Injection Cell comes with instructions on how to pack columns and is available as a stand alone unit (with 9 extra ferrules), or as a complete kit with ferrules, 1/8" stainless steel tubing, a spool of capillary, a cleaving tool, and a frit assembly, or a paritial kit.

Accurate Results

The Pressure Injection Cell is made with the finest methods and components, thereby enabling you to acquire very accurate data. The high quality stainless steel fittings and valve are made by Swagelok. The body and cap of the pressure chamber are nickel coated. Special features not found elsewhere include a recess in the cap to allow easy access to the sample tube and a hexagonal shape to help align the cap to the body. The Pressure Injection Cell's simple, rugged design and the use of top components ensure years of trouble free use and quality performance./p>

Load Samples into Mass Spectrometer

With the Pressure Injection Cell, load your sample into a mass spectrometer directly from a microcentrifuge or Eppenorf tube and avoid transfer loss. There is no contact with metallic surfaces which would adsorb negatively charged molecules.

Risk Free

The Pressure Injection Cell comes with a 30 day money back guarantee and a 2 year warranty

Operation

Operationally, the Pressure Injection Cell is analogous to a straw in a juice box. As you apply pressure in the box, the juice will be forced out through the straw. Likewise, the Pressure Injection Cell is connected to pressurized gas, typically in a tank. A 0.5 to 2 mL tube containing the sample liquid is placed in the base of the Pressure Injection Cell. A capillary is then placed through the ferrule in the cap and down into the sample tube. When packing a capillary, a frit assembly is placed at the distal end of the capillary to prevent particles from exiting and the entire Pressure Injection Cell is typically placed on a stir plat. By regulating the gas pressure, you can adjust the flow rate of the sample into the capillary.

Sample Data

On the left is a LC/MS chromatogram of a peptide mixture prepared from a tryptic digested gel band, separated by a 5µm C18 resin packed column (100 µm inside diameter by 10 cm long capillary). The Pressure Injection Cell ws used to pack the LC/MS capillary column and to inject the sample into the mass spectrometer. Courtesy of Dr Qishan Lin.

Schematic of System with Pressure Injection Cell

5 Models of Freedom Rocker™

Features

• Easy sample tube removal since the tube protrudes above the bottom section of the pressure injection cell.
• O-ring seal at interface ensures no leakage.
• Rated for 2500 psi (17,000kPa), ideal for consistent results with bottled gas.
• Available in 8500 psi (56,000 kPa) version
• Includes a 3-way valve for easy switching between pressurization and release of pressure
• Three bolt system secures the cell, allows easy addition and removal of tubes / vials
• Holds 0.5 to 2 ml microcentrifuge/Eppendorf tubes and 12 x 32 mm (glass) vials.
• Includes operator's manual; 10 reusable ferrules for typical (380 µm outside diameter) capillaries (other ferrules are available); and 1.5 mL of Kasil and 0.5 mL of formamide and a cleaving tool for making your own frits.
• Available with integrated magnetic stirplate.
• Warranty: 2 years parts and labor
• 30 day money back guarantee

Contacting our Customer Service Department--comments, questions, or anything can't find?

Call, e-mail, FAX, or type your comments below:

Contact details (optional, but required for us to reply or quote):

Name:		Organization/Country:
Email:		Phone/fax:

Regular business hours - 8:00 AM to 5:30 PM EST, M-F. E-mail replies normally expected within one business day.

PHONE: (908)788-5550;   FAX: (908)806-6631;   E-MAIL:

WEB: www.sisweb.com
ADDRESS: Scientific Instrument Services, 1027 Old York Rd., Ringoes, NJ 08551-1054.