|Overview||FAQ||Protocols||Beads and Accessories|
Below is a set of optimized protocols for the homogenization of various tissue, cell types, and organisms so you can spend less time troubleshooting and more time getting results.
All currently available protocols are listed below. Please read the General Guidelines before using the Bullet Blender® in any application.
|*Bullet Blender® 5 protocols: Use the protocols for the standard Bullet Blender and scale up as appropriate.
*Bullet Blender® 50-DX protocols: Scale up standard Bullet Blende protocols and use the following volumetric ration - 1:6:2 (sample:beads:buffer). Multiply the homogenized time by 3. It is recommended using 4.8 mm or 3.2 mm stainless steel beads for homogenization in 50 mL tubes.
|Types of samples||Protocols for Standard Bullet Blender®|
|Cell Culture||Candida albicans
Drosophila S2 Cells
Mammalian Cell Culture
Cancer Tissue (Carcinoma)
Fat / Adipose
Stomach / Jejunum
|Plant Tissue||A. Thaliana
Ginger Rhizome (Zingiber officinale)
Leek Leaves (Allium ampeloprasum)
Horseradish Root (Armoracia rusticana)
Malanga Tuber (Xanthosoma sagittifo
Pine Nut (Pinus)
Soy Bean (G. Max)
Sugar Cane Pulp (Saccharum)
|Small Organisms||Honeybee Apis mellifera
Roundworm C. Elegans
Zebrafish Danio Rerio
Fruit Fly (adult) Drosophila
Fruit Fly (larvae) Drosophila
|Extraction of Small Cells||Lymphocyte Extraction from Heart
Splenocyte Extraction from Speen
Bacterial Isolation from Organs
|Protocols for Use with Kits||QuickGene Mini80 - Tissue
QuickGene Mini80 - Cultured Cells
PLEASE NOTE: The protocols used with the Bullet Blender are different that protocols used with other bead-mill homogenizers. If you previously used another product, please be sure not to use its protocols with the Bullet Blender, as you will very likely experience different results.
Yttria coated filament at start
Yttria coated filament after 16,000 cycles