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140
Vials, Cryogenic
WHEATON 1.800.225.1437 (U.S. & Canada only)
|
+1.856.825.1100
|
+1.856.825.1368
(F)
| www.wheatonsci.com
Cryogenic Vials
Wheaton cryogenic vials, caps, vial racks, and freezer boxes are designed for simple identi-
fication and effortless handling. With Wheaton plastic Cryules
®
, both the closures and tubes
are manufactured from medical-grade polypropylene with the same coefficient of expan-
sion; this further enhances the leak proof qualities of these vials at changing temperatures.
All Wheaton Cryules and racks are autoclavable up to 121°C. When autoclaving plastic
Cryules, the cap must be loosened or removed to allow pressure equalization upon cooling;
this prevents pressure build-up within the vial.
The Wheaton glass Cryules are made from Wheaton 33 low extractable borosilicate glass,
(that conforms to ASTM Type I, Class A and USP Type I requirements) which can be
hermetically sealed.
Use caution; do not use cryogenic containers for storage directly in contact with the liquid
phase of liquid nitrogen; use in gas phase only. Always use appropriate safety equipment
such as gloves, face shields, and safety cabinets to protect personnel when removing
Cryules from cryogenic gases.
Cryogenic Vials, Freestanding
Cryule
®
Cryogenic Vial, Freestanding
Sterile
External threads
2 mL size
Supplied with colored coded caps
Conical bottom
These polypropylene vials are freestanding on the bench and lock into the rack
(Cat. No. 985810) for one-handed cap removal. The vertical inner wall and
conical bottom facilitate filling and maximum sample retrieval. The white marking
area and color coded caps allow for lot and sample identification. Vials are
packaged 50 per bag,10 bags per case.
Cat. No.
Cap Color
Size
(mL)
Dia x H
(mm)
Qty/Pack
Qty/Case
985734
White
2
12 x 46
50
500
985735
Red
2
12 x 46
50
500
985736
Pink
2
12 x 46
50
500
985737
Yellow
2
12 x 46
50
500
985738
Green
2
12 x 46
50
500
985739
Blue
2
12 x 46
50
500
•
•
•
•
•
Cryule
®
Cryogenic Vial, Freestanding
Sterile
Internal threads
Silicone ring sealing system
1.2, 2 and 4 mL size
These freestanding polypropylene vials are internally threaded. These vials and
caps feature a streamlined design with a silicone ring sealing system. Color
coded cap inserts can be used for lot identification. For cap inserts, see catalog
number series 242582. Vials are packaged 50 per bag,10 bags per case.
Cat. No.
Size
(mL)
Dia x H
(mm)
Qty/Pack
Qty/Case
985745
1.2
12 x 42
50
500
985746
2
12 x 49
50
500
985747
4
12 x 71
50
500
•
•
•
•
Cryule Vial with Colored Caps
Vial Rack for one-hand cap removal, 985810
Facts About Cryopreservation
Common Cryoprotectants
DMSO (dimethyl sulfoxide)
Ethylene glycol
Glycerol
Propylene glycol
Sucrose
Trehalose
•
•
•
•
•
•
Liquid nitrogen (LN
2
): liquid density at the triple point is
0.807 g/mL, is inert, colorless, odorless, non-corrosive,
nonflammable, and extremely cold.
Liquid nitrogen boils at -196°C, and is a cryogenic fluid
which is potentially capable of causing rapid frostbite on
contact with living tissue.
Cryopreservation is a process where cells or whole
tissues are preserved by cooling to low sub-zero
temperatures, such as (typically) 77 K or −196°C
(the boiling point of liquid nitrogen).
•
•
•
When cryoprotectant solutions are not used, the cells
being preserved are often damaged due to freezing
during the approach to low temperatures or warming to
room temperature.
A cryoprotectant is a substance that is used to protect
biological tissue from freezing damage (damage due
to ice formation).
Cryoprotectants act like antifreeze: they lower the
freezing temperature. They also increase the viscosity.
Instead of crystallizing, the syrupy solution turns into an
amorphous ice.
•
•
•
Freestanding Cryule Vial
Cap Inserts
See
Cap Inserts on page 142.
For
Cryogenic Ampules see the Ampule Section on page 1.
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