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Pages in this section: E5 E6 (all shown below)
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E5
Optimize Technologies Trap Cartridges
Scientific Instrument Services, Inc.
™
FAX: 908-806-6631
www.sisweb.com/catalog/08/E5
Trap Cartridges
O
ften a sample bound for HPLC or LC/MS analysis requires some type of clean-up to ensure optimal results. Detergents or salt can
affect the ionization process, while overly dilute samples can be swamped out by background noise, making them undetectable. There
are many methods to treat samples, but most require lengthy off-line procedures that involve significant sample handling. These pro-
cedures not only consume valuable time, but also can result in appreciable sample loss.
Optimize Technologies trap cartridges are an elegant and efficient means for sample processing. Trap cartridges are small packed beds that
use the adsorptive properties of analytes to effectively concentrate dilute samples, desalt, remove detergents, and more. Unlike off-line proce-
dures that require significant sample handling and potential sample loss, Optimize trap cartridges have the advantage of being able to be used in
a completely on-line environment. On-line clean up not only increased recovery, but is also amenable to automation for unattended use and high-
er throughput.
Traps work on the principle of “digital chromatography”. This is the most apt description because there are typically only two states of the ana-
lyte: bound and unbound. This on/off behavior is exploited by either trapping the analyte and letting sample matrix and contaminants go to waste;
or by trapping the undesirable components and allowing analytes to pass unimpeded. The discontinuous interaction of the analyte in a trap car-
tridge prevents dilution and dispersion that may occur in larger beds with a more distributive partitioning effect.
Optimize Technologies offers two lines of trap cartridges to suit any trapping need. The OPTI-PAK
™
capillary traps are designed for ultra-low
volume applications where any excess in volume could be detrimental to the assay. OPTI-LYNX
™
traps offer higher capacity, as well as the OPTI-
LYNX quick-connect hardware for the ultimate in connection simplicity. Both formats are available in a number of different phases and support
material, choose the trap design and material that best fits your needs. Optimize Technologies also offers custom dimensions and packings.
Trapping Applications
Though Optimize Technologies trap cartridges can be used for a number of different applications, two of the most
common are sample concentration/desalting and detergent removal. The general procedures listed below outline a
possible approach to these applications and illustrate the basic concepts to the trapping technique.
Sample Concentration/Desalting
Some samples contain an unacceptable concentration of salts and/or are in a volume that is too great for the
analytical technique being used. In these instances, a trap cartridge may be used to trap the analyte effectively
removing salts and eluting the analyte in a smaller, more concentrated volume of eluent.
To successfully concentrate and/or remove salts, the optimal strategy is that of trapping the analyte within the
trap cartridge and allowing undesirable components to go to waste. This can be done most efficiently by plumbing a
switching valve as shown in
Figure A.
The chemistry of the trap cartridge should be chosen such that there is good
selectivity for the analyte of interest, with little to no affinity for the salts or other contaminants. Loading should be
done with weak solvents to prevent the analyte from dispersing in the bed. Prudent selection of bed volumes can
also prevent unwanted sample dispersion. Optimize traps come in a variety of different volumes and dimensions to
facilitate the best trapping environment with minimal broadening or dispersion effects.
Once the sample has been sufficiently processed, the analyte is eluted from the trap. This can be done by run-
ning a gradient through the trap and onto an LC column, or it can be performed with a plug of a strong solvent elut-
ing the sample in one tight band. While the latter of these two options is best, it is not always possible. Using the
plumbing diagram from above, the elution valve position is displayed by
Figure B
.
Detergent Removal
The second strategy for trapping is exemplified by the application of detergent removal. Detergents may be pre-
sent as carryover from SDS PAGE analysis, or have been added to help solubilize the sample. Whatever the rea-
son, detergents greatly hinder MS analysis and, therefore, must be removed from the sample matrix. One of the most
common detergents is the ionic detergent sodium dodecyl sulfate (SDS). Fortunately, removal of SDS is a fairly sim-
ple task when using Optimize trap cartridges.
Ionic detergents like SDS can usually be removed using an ion exchange trap. SDS is an anionic detergent, so
a strong anion exchanger (SAX) is the packaging material of choice. The approach for detergent removal is exactly
the opposite of desalting/concentrating outlined above: the undesired component, i.e., the detergent is retained in the
trap cartridge while the analyte is flushed directly through to the LC column or MS. For the specific removal of SDS
from a protein mixture, it is best to keep the mobile phase pH at 4.4 or lower to keep the anionic amino acid
sidechains protonated and less likely to interact with the SAX functionalities.
One possible configuration of plumbing this set up is shown in
Figures C and D
. The first valve position puts the
gradient pump directly in-line with the trap, removing detergent from the sample matrix and sending detergent-free
matrix and analyte on for further analysis. The second position takes the trap out of the main flowpath so that it may
be regenerated for subsequent runs. With a judicious choice of loading and regenerating conditions, Optimize trap
cartridges can be reused numerous times.
Most trapping applications can be performed with the myriad of silica based packings we carry. For applications
requiring extreme pH, there is also the choice of polymeric packing materials. We also offer custom packing to ensure
that you will always have a solution available for your trapping and chromatographic needs.
Gradient Pump
Waste
Aux.
Pump
LC Column
Fig. A
Gradient Pump
Waste
Aux.
Pump
LC Column
Fig. B
Gradient Pump
Waste
Aux.
Pump
LC Column
Fig. D
Gradient Pump
Waste
Aux.
Pump
LC Column
Fig. C
background image
www.sisweb.com/catalog/08/E6
Optimize Technologies Trap Cartridges
E6
Scientific Instrument Services, Inc.
™
Phone: 908-788-5550
OPTI-LYNX
™
Trap Cartridges
B
uilt on the OPTI-LYNX quick-connect platform, OPTI-LYNX trap cartridges provide a versatile, easy-to-use cartridge based trap-
ping system. Each cartridge shares the same external dimensions, so that switching chemistries or capacities is only a matter
of a quarter-twist.
OPTI-LYNX traps are packed with special large particle silica and polymeric based materials to strike the perfect compromise between
sample capacity and backpressure. This choice of material gives OPTI-LYNX trap users a myriad of sample processing options. An OPTI-
LYNX trap cartridge may be set up in the loop of an injection or switching valve to facilitate on-line trapping. Alternatively, the large parti-
cle size is amenable to off-line, hand-loading applications. You can even combine the two: loading a trap off-line and then installing it in
your LC for elution directly into the solvent flowpath.
OPTI-PAK
™
Capillary Trap Cartridges
T
he OPTI-PAK Capillary Trap Cartridge utilizes the ultimate in engineering and fluidic design to deliver a trap column that is both
easy to use, and extremely effective.The OPTI-PAK is specially designed for use in today’s low volume applications. With bed
volumes ranging from 0.12 µL to 5.0 µL, you can be sure to find the perfect balance between sample capacity and trap volume.
A packed stem, enabled by a proprietary method of frit placement, places the packed bed directly into the flowpath, eliminating all excess
swept volume. Less swept volume translates to an absolute minimum in sample dilution and dispersion. The unique hardware design inte-
grates a PEEK holder with an auto-adjusting stem that fluidly interfaces to any 10-32 port, providing a zero-dead-volume connection no
matter what the tube stop depth.
Sorbent
Part No.
Bed
C18
C18AQ
C8
C4
SCX
SAX
DVB
10-03328-
**
0.12 µL
10-03328TA Part #1003328TA--Click to order
Current Price: $579.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
10-03328TB Part #1003328TB--Click to order
Current Price: $579.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
10-03328TC Part #1003328TC--Click to order
Current Price: $579.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
10-03328TD Part #1003328TD--Click to order
Current Price: $579.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
10-03328TE Part #1003328TE--Click to order
Current Price: $579.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
10-03328TF Part #1003328TF--Click to order
Current Price: $579.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
10-03328TG
10-03324-
**
0.25 µL
10-03324TA Part #1003324TA--Click to order
Current Price: $556.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
10-03324TB Part #1003324TB--Click to order
Current Price: $556.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
10-03324TC Part #1003324TC--Click to order
Current Price: $556.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
10-03324TD Part #1003324TD--Click to order
Current Price: $556.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
10-03324TE Part #1003324TE--Click to order
Current Price: $556.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
10-03324TF Part #1003324TF--Click to order
Current Price: $556.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
10-03324TG Part #1003324TG--Click to order
Current Price: $556.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
10-03317-
**
0.50 µL
10-03317TA Part #1003317TA--Click to order
Current Price: $436.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
10-03317TB Part #1003317TB--Click to order
Current Price: $436.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
10-03317TC Part #1003317TC--Click to order
Current Price: $436.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
10-03317TD Part #1003317TD--Click to order
Current Price: $436.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
10-03317TE
10-03317TF Part #1003317TF--Click to order
Current Price: $436.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
10-03317TG
10-03225-
**
1.00 µL
10-03225TA Part #1003225TA--Click to order
Current Price: $436.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
10-03225TB Part #1003225TB--Click to order
Current Price: $436.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
10-03225TC Part #1003225TC--Click to order
Current Price: $436.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
10-03225TD Part #1003225TD--Click to order
Current Price: $436.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
10-03225TE Part #1003225TE--Click to order
Current Price: $436.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
10-03225TF Part #1003225TF--Click to order
Current Price: $436.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
10-03225TG Part #1003225TG--Click to order
Current Price: $436.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
10-03229-
**
2.00 µL
10-03229TA Part #1003229TA--Click to order
Current Price: $436.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
10-03229TB Part #1003229TB--Click to order
Current Price: $436.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
10-03229TC Part #1003229TC--Click to order
Current Price: $436.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
10-03229TD Part #1003229TD--Click to order
Current Price: $436.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
10-03229TE Part #1003229TE--Click to order
Current Price: $436.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
10-03229TF Part #1003229TF--Click to order
Current Price: $436.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
10-03229TG Part #1003229TG--Click to order
Current Price: $436.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
10-03233-
**
5.00 µL
10-03233TA Part #1003233TA--Click to order
Current Price: $436.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
10-03233TB Part #1003233TB--Click to order
Current Price: $436.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
10-03233TC Part #1003233TC--Click to order
Current Price: $436.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
10-03233TD Part #1003233TD--Click to order
Current Price: $436.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
10-03233TE Part #1003233TE--Click to order
Current Price: $436.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
10-03233TF Part #1003233TF--Click to order
Current Price: $436.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
10-03233TG Part #1003233TG--Click to order
Current Price: $436.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
Two Letter Packing CodeTA
TB
TC
TD
TE
*Use the trap cartridge code table above to find the seven digits that match the bed/volume you require. then find the two letter packing code for your sorbent phase.
Example: a five pack of 0.5 µL trap cartridges with SCX sorbent would have a part number 10-03317-TE.
OPTI-PAK™Trap Cartridges, 5pk
Part No.
Description
11-02225-AA Part #1102225AA--Click to order
Current Price: $200.00
Unit of Measure: EA
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
Opti-Lynx In-Line Trap Cartridge Holder
Includes: Bayonet Connector, holder cap/tube, fittings
(Trap Cartridges are sold separately - use table below
to find your part number.)
10-20-02877 Part #102002877--Click to order
Current Price: $61.00
Unit of Measure: EA
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
Tube Set, for OPTI-LYNX Trap Cartridges
(Rheodyne Injection Valve)*
10-20-02878 Part #102002878--Click to order
Current Price: $46.00
Unit of Measure: EA
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
Tube Set, for OPTI-LYNX Trap Cartridges
(Valco Injection Valve)*
*These tube sets come with two 30 cm lengths of 0.003" ID PEEK tubing and 2 valve port fittings designed for use with
the specified injection valve. Can also be used for making in-line connections.
Sorbent
Part No.
Bed
C18
SCX
C18AQ
SAX
C8
C4
DVB
11-02868-
**
4 µL
11-02868TA Part #1102868TA--Click to order
Current Price: $378.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
11-02868TB Part #1102868TB--Click to order
Current Price: $378.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
11-02868TD Part #1102868TD--Click to order
Current Price: $378.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
11-02868TE Part #1102868TE--Click to order
Current Price: $378.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
11-02868TF Part #1102868TF--Click to order
Current Price: $378.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
11-02868TG Part #1102868TG--Click to order
Current Price: $378.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
11-02868TH Part #1102868TH--Click to order
Current Price: $390.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
11-02870-
**
20 µL
11-02870TA Part #1102870TA--Click to order
Current Price: $378.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
11-02870TB Part #1102870TB--Click to order
Current Price: $378.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
11-02870TD Part #1102870TD--Click to order
Current Price: $378.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
11-02870TE Part #1102870TE--Click to order
Current Price: $378.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
11-02870TF Part #1102870TF--Click to order
Current Price: $378.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
11-02870TG Part #1102870TG--Click to order
Current Price: $378.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
11-02870TH Part #1102870TH--Click to order
Current Price: $390.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
11-02872-
**
40 µL
11-02872TA Part #1102872TA--Click to order
Current Price: $378.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
11-02872TB Part #1102872TB--Click to order
Current Price: $378.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
11-02872TD Part #1102872TD--Click to order
Current Price: $378.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
11-02872TE Part #1102872TE--Click to order
Current Price: $378.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
11-02872TF Part #1102872TF--Click to order
Current Price: $378.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
11-02872TG Part #1102872TG--Click to order
Current Price: $378.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
11-02872TH Part #1102872TH--Click to order
Current Price: $390.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
11-02874-
**
100 µL
11-02874TA Part #1102874TA--Click to order
Current Price: $378.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
11-02874TB Part #1102874TB--Click to order
Current Price: $378.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
11-02874TD Part #1102874TD--Click to order
Current Price: $378.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
11-02874TE Part #1102874TE--Click to order
Current Price: $378.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
11-02874TF Part #1102874TF--Click to order
Current Price: $378.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
11-02874TG Part #1102874TG--Click to order
Current Price: $378.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
11-02874TH Part #1102874TH--Click to order
Current Price: $390.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
Two Letter Packing Code
TA
TB
TD
TE
*Use the trap cartridge code table above to find the seven digits that match the bed/volume you require. then find the two letter packing code for your sorbent phase.
Example: a five pack of 20 µL trap cartridges with SCX sorbent would have a part number 11-02870-TB Part #1102870TB--Click to order
Current Price: $378.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
.
OPTI-LYNX™Trap Cartridges, 5pk
OPTI-LYNX Trap Holder Hardware
Optimize Technologies special-
izes in custom packing solu-
tions. Please contact us if you
do not see the phase/sorbents
you require.
Optimize Technologies special-
izes in custom packing solu-
tions. Please contact us if you
do not see the materials you
require.
Part No.
Description
10-20-02875 Part #102002875--Click to order
Current Price: $44.00
Unit of Measure: EA
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
Tube Set, for OPTI-PAK Trap Cartridges
(Rheodyne Injection Valve)*
10-20-02876 Part #102002876--Click to order
Current Price: $31.00
Unit of Measure: EA
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
Tube Set, for OPTI-PAK Trap Cartridges
(Valco Injection Valve)*
*These tube sets come with one 30 cm length of 0.003" ID PEEK tubing and a valve port fitting designed for use with
the specified injection valve. Can also be used for making in-line connections.
OPTI-LYNX™Trap Holder Hardware
TF
TF
TG
TH
TG
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