Electrospray Tips from New Objective

Introduction:

Bring Electrospray into Focus with a New Objective PicoTip! Break the 1 µl/min barrier. Upgrade your conventional ESI emitter to a New Objective glass or fused silica PicoTip™. Scanning Electronic Microscopy reveals the PicoTip secret: A microfabricated structure perfect for low-flow rate ESI-MS. Unlike some products from other suppliers, there is no need to break open or grind PicoTips™ prior to use.

Product Line:

Fused Silica 

Use small OD (< 1 mm) fused-silica tips for online, low-flow ESI methods such as: sample introduction with injection valves, auto-samplers,capillary HPLC, CE or CEC. 

Specifications:

• 150, 360, or 670 µm OD tubing; <2 to >30 µm tip ID's
• Flow rates from <10 nl/min to >500 nl/min
• Premium multi-layer conductive coatings (U.S. Patent 5,788,166).
• Lower chemical noise than glass tips
• New! Distal coatings for implementing liquid junction contact with polymer HPLC hardware.

Glass or Quartz

Use large OD (> or = 1 mm) glass or quartz tips for traditional "off-line" nano-electrospray methods. Our glass tips have a well defined orifice for easy ESI initiation with a minimum of sample loss. No need to break open prior to use.

Specifications:

• 1.0 or 1.2 mm OD tubing.
•  "Omega dot" tubing for efficient capillary filling.
• Select from: -Premium glass or quartz PicoTips ™ individually inspected, multi-layer coatings, tip ID's 2 to 4 µm -New! Inexpensive glass EconoTips 1.0 p.m tip ID and a conventional conductive coating.

Sharp, burr free silica tips are perfect for nano-LC/MS and sheathless CE/MS.

The 10 µm ID, over-coated tip on the left was pumped (200 nL/min) with 30% MeOH, 5% Acetic Acid. Note the clean, stable Taylor cone and spray jet @ 1200 V (5 mm counter-electrode distance).

A Variety of Tips to Suit Your Application 

Ask us about custom orders!

Wide-bore and narrow-bore Picotips™ can be nested to create versatile, ultra-low volume ESI emitters. Here, a 30 µm tip fabricated from 360 µm OD tubing has been inserted into a 15 µm tip made from 450 µm ID (670 OD) fused silica tubing.

Patented Multi-layer Conductive Coatings

New Objective uses a patented (U.S. Patent 5,788,166), multi-layer coating process. Our pure gold layer is overcoated with an inert, electrochemically stable dieletric layer, locking the gold into place. The resulting coating has excellent resistance to ESI solvent exposure and exceptional electrochemical stability. To try and improve coating performance, other manufacturers increase the mechanical durability of the gold layer by introducing alloy or inter-layer metals. During handling the coating may appear to be more durable, but it may not yield improved ESI performance. These alloy and inter-layer metals can actually reduce the coating's electrochemical stability resulting in coating degradation at the tip.

Stability With Stop/Start Performance

The figure on the left shows the stability of signal at three time scales. The modulation in current is due to turning the spray voltage on and off. Note how the ion current returns to its former level. This tip was sprayed without failure for over 3 hours and was "retired" still functional. The total ion current is (through 3 RF-only quadrupoles) from a 360, 50 (ID/OD) needle with a 5 µm ID tip, operating at 60 nL/min spraying 20 µM Ubiquitin in 2% HoAC.

Glass Pico Tip Standard Dimensions: 1.0 mm OD Tubing: ca. 5 cm long, coating length 3.5 to 4 cm.1.0 mm  Tips are compatible with Micromass Systems 1.2 &1.5 OD Tubing: ca. 5 cm long, coating length 1.5 to 2 cm 1.2 mm  Tips are compatible with Protana (PAC), Bruker, & PE BioSystems

Fused Silica Pico Tip Standard Dimensions: Tip-end coating: Tip length: ca. 5 cm long, coating length 1.5 to 2 cm. Distal-end coating: Tip length: ca. 5 cm long, coating length 3 to 3.5 cm from distal end.

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