New Objective PicoFrit
®
Columns
E46
Scientific Instrument Services, Inc.
TM
Phone: 908-788-5550
www.sisweb.com/catalog/08/E46
PicoFrit Columns
PicoFrit Benefits:
- Eliminates band broadening
- Sprays directly from column to inlet
- Minimizes post-column loss
- Integral frit greatly reduces tip clogging
- Robust performance compatible with automated systems
- High-resolution separations yield femtomole sensitivity
*Frit material is borosilicate glass; silica available on a custom
basis.
The PicoFrit Advantage
Spraying directly off the column boosts the performance of your
mass spectrometer compared to that provided by a column
attached to a tip. Less time spent making connections increases
efficiency. That's the PicoFrit advantage.
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Ion chromatograms for full-scan MS data of 4 angiotensin
variants at 25 fmol injected on column
-
The chromatogram at left was obtained using a C18-packed
column coupled to a coated emitter with a union
-
The chromatogram at right was obtained using a C18-packed
PicoFrit column (75 µm OD/15 µm ID)
-
The PicoFrit column yielded better peak shape with
decreased peak width, resulting in higher sensitivity
PicoFrit Specifications
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Column IDs 75 µm and 50 µm, tip IDs of 15 µm and 10 µm
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Packed with a variety of high-performance, reverse-phase,
HPLC packings
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Short columns (1 cm) for sample concentration/desalting
procedures
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Medium columns (5 cm) for rapid-gradient LC-MS
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Long columns (10 cm) for gradient LC-MS
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Biphasic columns for shotgun digests
The PicoFrit Robustness
The study of global proteomics requires robust performance on
a 24/7 basis. With the right sample preparation, PicoFrit® columns
provide high predictability without compromising sensitivity.
Standard Column Coupled
PicoFrit
TM
Column
with a Tip
Injection #31 - DAY TWO
Plasma membrane challenged with insulin
In gel digestion of slice no. 30 of 64
75 µm 4x10 cm C18 PicoFrit® column with 300 µm,
1 mm C18 Trap Cartridge on Micromass® Q-ToFTM
Injection #881 - ONE MONTH LATER
Smooth endoplasmic reticulum, aqueous phase
In gel digestion of slice no. 45 of 92
Over 30 days of continuous, 24/7 operation
tip...
