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Pages in this section: E46 E47 E48 E49 (all shown below)
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New Objective PicoFrit
®
Columns
E46
Scientific Instrument Services, Inc.
™
Phone: 908-788-5550
www.sisweb.com/catalog/08/E46
PicoFrit Columns
PicoFrit Benefits:
• Eliminates band broadening
• Sprays directly from column to inlet
• Minimizes post-column loss
• Integral frit greatly reduces tip clogging
• Robust performance compatible with automated systems
• High-resolution separations yield femtomole sensitivity
*Frit material is borosilicate glass; silica available on a custom
basis.
The PicoFrit Advantage
Spraying directly off the column boosts the performance of your
mass spectrometer compared to that provided by a column
attached to a tip. Less time spent making connections increases
efficiency. That's the PicoFrit advantage.
•
Ion chromatograms for full-scan MS data of 4 angiotensin
variants at 25 fmol injected on column
•
The chromatogram at left was obtained using a C18-packed
column coupled to a coated emitter with a union
•
The chromatogram at right was obtained using a C18-packed
PicoFrit column (75 µm OD/15 µm ID)
•
The PicoFrit column yielded better peak shape with
decreased peak width, resulting in higher sensitivity
PicoFrit Specifications
•
Column IDs 75 µm and 50 µm, tip IDs of 15 µm and 10 µm
•
Packed with a variety of high-performance, reverse-phase,
HPLC packings
•
Short columns (1 cm) for sample concentration/desalting
procedures
•
Medium columns (5 cm) for rapid-gradient LC-MS
•
Long columns (10 cm) for gradient LC-MS
•
Biphasic columns for shotgun digests
The PicoFrit Robustness
The study of global proteomics requires robust performance on
a 24/7 basis. With the right sample preparation, PicoFrit® columns
provide high predictability without compromising sensitivity.
Standard Column Coupled
PicoFrit
™
Column
with a Tip
Injection #31 - DAY TWO
Plasma membrane challenged with insulin
In gel digestion of slice no. 30 of 64
75 µm 4x10 cm C18 PicoFrit® column with 300 µm,
1 mm C18 Trap Cartridge on Micromass® Q-ToF™
Injection #881 - ONE MONTH LATER
Smooth endoplasmic reticulum, aqueous phase
In gel digestion of slice no. 45 of 92
Over 30 days of continuous, 24/7 operation
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E47
New Objective PicoFrit
®
Columns
Scientific Instrument Services, Inc.
™
FAX: 908-806-6631
www.sisweb.com/catalog/08/E47
Packing
Part
Column
Tip Size
Bed Length
Pieces per
Material
No.
ID (µm)
(µm)
(cm)
Pkg.
ProteoPep
™
II
PFC7515-PP2-1
75
15
1
1
PFC7515-PP2-1-4PK
75
15
1
4
PFC7515-PP2-5
75
15
5
1
PFC7515-PP2-5-3PK
75
15
5
3
PFC7515-PP2-10 Part #PFC7515PP210--Click to request quote
DISCONTINUED
Unit of Measure: EA
75
15
10
1
PFC7515-PP2-10-3PK
75
15
10
3
PFC5010-PP2-1
50
10
1
1
PFC5010-PP2-1-4PK
50
10
1
4
PFC5010-PP2-5
50
10
5
1
PFC5010-PP2-5-3PK
50
10
5
3
PFC5010-PP2-10
50
10
10
1
PFC5010-PP2-10-3PK
50
10
10
3
ProteoPep
™
PFC7515-PP18-1
75
15
1
1
PFC7515-PP18-1-4PK
75
15
1
4
PFC7515-PP18-5 Part #PFC7515PP185--Click to request quote
DISCONTINUED
Unit of Measure: EA
75
15
5
1
PFC7515-PP18-5-3PK
75
15
5
3
PFC7515-PP18-10
75
15
10
1
PFC7515-PP18-10-3PK
75
15
10
3
PFC5010-PP18-1
50
10
1
1
PFC5010-PP18-1-4PK
50
10
1
4
PFC5010-PP18-5
50
10
5
1
PFC5010-PP18-5-3PK
50
10
5
3
PFC5010-PP18-10
50
10
10
1
PFC5010-PP18-10-3PK
50
10
10
3
AQUASIL
™
PFC7515-AQ-1
75
15
1
1
PFC7515-AQ-1-4PK
75
15
1
4
PFC7515-AQ-5
75
15
5
1
PFC7515-AQ-5-3PK Part #PFC7515AQ53PK--Click to order
Current Price: $745.00
Unit of Measure: EA
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
75
15
5
3
PicoFrit Columns
Premier Chemistries
PROTEOPEP
™
II
ProteoPep II produces sharper peaks with less noise than ProteoPep.
•
C18, 300 Å pore material excellent for both hydrophobic and
hydrophilic peptides
•
Exceptional peak shape for peptide analysis with trace
(0.005%) or no TFA
•
High peak counts — high resolution
•
Peptide mapping and purification
•
Synthetic peptide purification
•
5 µm particle size
Standard Chemistries
ProteoPep
•
C18, 300 Å
•
Exceptional peak shape for peptide analysis with trace
(0.005%) or no TFA
•
Recommended for intact proteins
•
5 µm particle size
BioBasic
®
•
C18, C8 and C4, 300 Å
•
Highly deactivated
•
Ideal for proteins and peptides
•
Extremely stable bonding chemistry
•
5 µm particle size
AQUASIL
®
•
C18, 100 Å
•
Immune to retention loss by pure water
•
Deactivated acids or bases
•
Good performance with low TFA
•
5 µm particle size
Biphasic PicoFrit® Columns
•
Analyzes hundreds of proteins in a single run
•
Combines high-quality cation exchange material with
ProteoPep™ II C18 or any of our other premier chemistries
Packed PicoFrit Columns
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New Objective PicoFrit
®
Columns
E48
Scientific Instrument Services, Inc.
™
Phone: 908-788-5550
www.sisweb.com/catalog/08/E48
AQUASIL
™
(cont.)
PFC7515-AQ-10
75
15
10
1
PFC7515-AQ-10-3PK Part #PFC7515AQ103PK--Click to request quote
DISCONTINUED
Unit of Measure: PK
75
15
10
3
PFC5010-AQ-1
50
10
1
1
PFC5010-AQ-1-4PK
50
10
1
4
PFC5010-AQ-5
50
10
5
1
PFC5010-AQ-5-3PK
50
10
5
3
PFC5010-AQ-10
50
10
10
1
PFC5010-AQ-10-3PK
50
10
10
3
BioBasic
®
C18
PFC7515-BI-1
75
15
1
1
PFC7515-BI-1-4PK
75
15
1
4
PFC7515-BI-5
75
15
5
1
PFC7515-BI-5-3PK Part #PFC7515BI53PK--Click to request quote
DISCONTINUED
Unit of Measure: PK
75
15
5
3
PFC7515-BI-10 Part #PFC7515BI10--Click to request quote
DISCONTINUED
Unit of Measure: EA
75
15
10
1
PFC7515-BI-10-3PK Part #PFC7515BI103PK--Click to request quote
DISCONTINUED
Unit of Measure: PK
75
15
10
3
PFC5010-BI-1
50
10
1
1
PFC5010-BI-1-4PK
50
10
1
4
PFC5010-BI-5
50
10
5
1
PFC5010-BI-5-3PK
50
10
5
3
PFC5010-BI-10
50
10
10
1
PFC5010-BI-10-3PK Part #PFC5010BI103PK--Click to order
Current Price: $0.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
50
10
10
3
BioBasic
®
C8
PFC7515-BI8-1
75
15
1
1
PFC7515-BI8-1-4PK
75
15
1
4
PFC7515-BI8-5
75
15
5
1
PFC7515-BI8-5-3PK
75
15
5
3
PFC7515-BI8-10
75
15
10
1
PFC7515-BI8-10-3PK
75
15
10
3
PFC5010-BI8-1
50
10
1
1
PFC5010-BI8-1-4PK
50
10
1
4
PFC5010-BI8-5
50
10
5
1
PFC5010-BI8-5-3PK
50
10
5
3
PFC5010-BI8-10
50
10
10
1
PFC5010-BI8-10-3PK
50
10
10
3
BioBasic
®
C4
PFC7515-BI4-1
75
15
1
1
PFC7515-BI4-1-4PK
75
15
1
4
PFC7515-BI4-5
75
15
5
1
PFC7515-BI4-5-3PK
75
15
5
3
PFC7515-BI4-10
75
15
10
1
PFC7515-BI4-10-3PK
75
15
10
3
PFC5010-BI4-1
50
10
1
1
PFC5010-BI4-1-4PK
50
10
1
4
PFC5010-BI4-5
50
10
5
1
PFC5010-BI4-5-3PK
50
10
5
3
PFC5010-BI4-10
50
10
10
1
PFC5010-BI4-10-3PK
50
10
10
3
Strong Cation Exchange
PFC7515-CX-1
75
15
1
1
PFC7515-CX-1-4PK
75
15
1
4
PFC7515-CX-5
75
15
5
1
PFC7515-CX-5-3PK
75
15
5
3
PFC7515-CX-10
75
15
10
1
PFC7515-CX-10-3PK
75
15
10
3
PFC5010-CX-1
50
10
1
1
PFC5010-CX-1-4PK
50
10
1
4
PFC5010-CX-5
50
10
5
1
PFC5010-CX-5-3PK
50
10
5
3
PFC5010-CX-10
50
10
10
1
PFC5010-CX-10-3PK
50
10
10
3
Packing
Part
Column
Tip Size
Bed Length
Pieces per
Material
No.
ID (µm)
(µm)
(cm)
Pkg.
Packed PicoFrit Columns (cont.)
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E49
New Objective PicoFrit
®
Columns
Scientific Instrument Services, Inc.
™
FAX: 908-806-6631
www.sisweb.com/catalog/08/E49
Self- Pack PicoFrits
Ready for loading - Perfect for Proteomics
PicoFrit
®
columns are ready for you to pack with your preferred polymer or silica media. Column packing
is made easy by the low back-pressure frit.
•
Spray directly from column to inlet
•
Minimize post-column loss
Part Number
Tubing Size
Nominal Tip
Frit Length
Tubing Length
Pieces per
(OD/ID µm)
ID (µm)
(µm)
(cm)
Pkg.
Standard Coating
PF360-75-30-CE-5 Part #PF3607530CE5--Click to order
Current Price: $415.00
Unit of Measure: EA
Est. Ship Weight=1 lbs
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
360/75
30 ± 2.0
≤ 350
50
5
PF360-75-15-CE-5 Part #PF3607515CE5--Click to order
Current Price: $415.00
Unit of Measure: EA
Est. Ship Weight=1 lbs
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
360/75
15 ± 1.5
≤ 250
50
5
PF360-75-10-CE-5 Part #PF3607510CE5--Click to order
Current Price: $415.00
Unit of Measure: EA
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
360/75
10 ±1.0
≤ 200
50
5
PF360-50-30-CE-5 Part #PF3605030CE5--Click to order
Current Price: $415.00
Unit of Measure: EA
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
360/50
30 ± 2.0
≤ 350
50
5
PF360-50-15-CE-5 Part #PF3605015CE5--Click to order
Current Price: $415.00
Unit of Measure: EA
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
360/50
15 ±1.5
≤ 250
50
5
PF360-50-10-CE-5 Part #PF3605010CE5--Click to order
Current Price: $415.00
Unit of Measure: EA
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
360/50
10 ±1.0
≤ 200
50
5
No Coating
PF360-75-30-N-5 Part #PF3607530N5--Click to order
Current Price: $375.00
Unit of Measure: EA
Est. Ship Weight=1 lbs
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
360/75
30 ± 2.0
≤350
50
5
PF360-75-15-N-5 Part #PF3607515N5--Click to order
Current Price: $375.00
Unit of Measure: EA
Est. Ship Weight=1 lbs
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
360/75
15 ±1.5
≤ 250
50
5
PF360-75-10-N-5 Part #PF3607510N5--Click to order
Current Price: $375.00
Unit of Measure: EA
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
360/75
8 ±1.0
≤ 200
50
5
PF360-50-30-N-5 Part #PF3605030N5--Click to order
Current Price: $375.00
Unit of Measure: EA
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
360/50
30 ± 2.0
≤350
50
5
PF360-50-15-N-5 Part #PF3605015N5--Click to order
Current Price: $375.00
Unit of Measure: EA
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
360/50
15 ±1.5
≤ 250
50
5
PF360-50-10-N-5 Part #PF3605010N5--Click to order
Current Price: $375.00
Unit of Measure: EA
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
360/50
8 ±1.0
≤ 200
50
5
Self-Pack PicoFrit
®
Assortment Kits -
Includes: (1) PF360-75-8, (2) PF360-75-15, and (2) PF360-75-30
FRITKIT-360-CE Part #FRITKIT360CE--Click to order
Current Price: $415.00
Unit of Measure: EA
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
Standard Coating
FRITKIT-360-N Part #FRITKIT360N--Click to order
Current Price: $375.00
Unit of Measure: EA
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
No Coating
Self-Pack PicoFrits
®
Ordering Information
Biphasic PicoFrit Columns
Biphasic PicoFrit
®
columns combine ProteoPep
™
II C18 chemistry with strong cation exchange.
Part
Column
Tip Size
Bed Length
Pieces per
No.
ID (µm)
(µm)
(cm)
Pkg.
PFC7515-CXPP2-10 Part #PFC7515CXPP210--Click to order
Current Price: $0.00
Unit of Measure: EA
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
75
15
10
1
PFC7515-CXPP2-10-3
75
15
10
3
PFC5010-CXPP2-10 Part #PFC5010CXPP210--Click to order
Current Price: $0.00
Unit of Measure: EA
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
50
10
10
1
PFC5010-CXPP2-10-3 Part #PFC5010CXPP2103--Click to order
Current Price: $0.00
Unit of Measure: PK
Not in stock. Standard lead time for out-of-stock items is approximately two weeks.
50
10
10
3
Related information may be found on the following pages of our web site:
PageParts
New Objective PicoFrit Electrospray TipsPF3607515CE5, PF3607515N5, PF3607530CE5, PF3607530N5
Page Number: [ previous page next page ]
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